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A lens-specific co-injection marker for medaka transgenesis

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    0346841 - ÚMG 2011 RIV US eng J - Journal Article
    Vopálenský, Pavel - Růžičková, Jana - Pavlů, Barbora - Kozmik, Zbyněk
    A lens-specific co-injection marker for medaka transgenesis.
    Biotechniques. Roč. 48, č. 3 (2010), s. 235-236. ISSN 0736-6205. E-ISSN 1940-9818
    R&D Projects: GA AV ČR IAA500520908; GA MŠMT 1M0520
    Grant - others:GA UK(CZ) 91808
    Institutional research plan: CEZ:AV0Z50520514
    Keywords : lens * transgenic * crystallin
    Subject RIV: EB - Genetics ; Molecular Biology
    Impact factor: 2.550, year: 2010

    Recent advances in fish transgenesis allow generation of transgenic animals at high numbers and efficiency of germ-line transmission. To facilitate the procedure, a suitable co-injection marker may be very helpful for preselection of transgenic embryos. For this purpose, a lens-specific marker appears to be a suitable candidate since the lens is a well defined tissue positioned at the periphery and is easily accessible for examination of reporter gene expression. Here, we constructed reporter vectors based on mouse gamma-F crystallin (mF-Cry) promoter driving heterologous expression of the reporter gene at a high level and very specifically to the lens allowing an easy sorting of transgenics.
    Permanent Link: http://hdl.handle.net/11104/0187759

     
     
Number of the records: 1  

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