Ribonucleoprotein localization in mouse oocytes

0346833 - ÚMG 2011 RIV US eng J - Journal Article
Flemr, Matyáš - Svoboda, Petr
Ribonucleoprotein localization in mouse oocytes.
Methods. Roč. 53, č. 2 (2011), s. 136-141. ISSN 1046-2023. E-ISSN 1095-9130
R&D Projects: GA ČR GA204/09/0085; GA ČR GAP305/10/2215; GA MŠMT ME09039
Institutional research plan: CEZ:AV0Z50520514
Keywords : mouse oocyte * in situ hybridization * immunofluorescence
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.011, year: 2011

RNA molecules rarely function alone in cells. For most RNAs, their function requires formation of various ribonucleoprotein (RNP) complexes. For example, mRNP composition can determine mRNA localization, translational repression, level of translation or mRNA stability. RNPs are usually studied by biochemical methods. However, biochemical approaches are unsuitable for some model systems, such as mammalian oocytes and early embryos, due to the small amounts that can be obtained for experimental analysis. In such cases, microscopic techniques are often used to learn about RNPs. Here, we present a review of immunostaining, fluorescence in situ hybridization with subcellular resolution and a combination of both, with emphasis on the mouse oocyte and early embryos models. Application of these techniques to whole-mount fixed oocytes and early embryos can provide information about RNP composition and localization with three-dimensional resolution.
Permanent Link: http://hdl.handle.net/11104/0187752