Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells

0346830 - ÚMG 2011 RIV GB eng J - Journal Article
Blume, Y. - Yemets, A. - Sheremet, Y. - Nyporko, A. - Sulimenko, Vadym - Sulimenko, Tetyana - Dráber, Pavel
Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells.
BMC Plant Biology. Roč. 10, x (2010), s. 29-29. ISSN 1471-2229. E-ISSN 1471-2229
R&D Projects: GA MŠMT LC545; GA AV ČR KAN200520701
Grant - others:EU-INTAS(XE) 03-51-6459
Institutional research plan: CEZ:AV0Z50520514
Keywords : beta-tubulin * plant microtubule * epitope
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.085, year: 2010

This research article reports on the correlation between localization of five small beta-tubulin regions on A. thaliana microtubule protofilament model and their exposure on cortical microtubules in cells. Using homology modeling it was generated the model for the prediction of surface exposure beta-tubulin epitopes and tyrosine residues. Peptide scans disclosed the position of epitopes and following verification of the results was done by immunofluorescence microscopy concerning that beta-tubulins could be one of the targets for tyrosine kinases. This article can contribute to a better understanding of the interactions of plant microtubules with associated proteins.
Permanent Link: http://hdl.handle.net/11104/0187749