A new type of membrane raft-like microdomains and their possible involvement in TCR signaling

0346793 - ÚMG 2011 RIV US eng J - Journal Article
Otáhal, Pavel - Angelisová, Pavla - Hrdinka, Matouš - Brdička, Tomáš - Novák, Petr - Drbal, Karel - Hořejší, Václav
A new type of membrane raft-like microdomains and their possible involvement in TCR signaling.
Journal of Immunology. Roč. 184, č. 7 (2010), s. 3689-3696. ISSN 0022-1767. E-ISSN 1550-6606
Institutional research plan: CEZ:AV0Z50520514; CEZ:AV0Z50200510
Keywords : membrane rafts * LAT * T-receptor
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 5.745, year: 2010

One of the key signaling molecules present in T cell detergent-resistant membrane domains (DRMs) derived from membrane rafts is the transmembrane adaptor protein LAT (linker for activation of T cells). In contrast to previous results, a recent study demonstrated that a LAT construct not present in the buoyant DRMs is fully able to support TCR signaling and development of T cells in vivo. This finding caused doubts about the real physiological role of rafts in TCR signaling. In this study, we demonstrate that these results can be explained by the existence of a novel type of membrane raft-like microdomains, containing a number of membrane molecules. At a moderate level of expression, LAT supported TCR signaling more efficiently than constructs targeted to the microdomains producing heavy DRMs or to nonraft membrane. We suggest that different types of membrane microdomains provide environments regulating the functional efficiencies of signaling molecules present therein.
Permanent Link: http://hdl.handle.net/11104/0187721