Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas

0346752 - ÚMG 2011 RIV US eng J - Journal Article
Plachý, Jiří - Kotáb, Jan - Divina, Petr - Reinišová, Markéta - Šenigl, Filip - Hejnar, Jiří
Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas.
Journal of Virology. Roč. 84, č. 9 (2010), s. 4204-4211. ISSN 0022-538X. E-ISSN 1098-5514
R&D Projects: GA ČR(CZ) GA204/07/1030; GA ČR GP301/09/P667
Institutional research plan: CEZ:AV0Z50520514
Keywords : avian sarcoma virus * retrovirus integration * position effects
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 5.189, year: 2010

Retroviruses and retrovirus-derived vectors integrate non-randomly into the genomes of host cells with specific preferences for transcribed genes, gene-rich regions, and CpG islands. To understand the genomic features that influence transcriptinal activity of integrated retroviruses, we cloned and analyzed the integration sites of avian sarcoma virus from chicken tumors. Growing progressively in dependence on high and stable expression of the transduced v-src oncogene, these tumors represent clonal expansions of cells bearing transcriptionally active replication-defective proviruses. Our analysis showed striking accumulation within or close to transcription units, particularly in genes broadly expressed in multiple tissues but not in tissue-specifically expressed genes. We infer that proviruses integrated in these genomic areas efficiently avoid transcriptional silencing and keep active for a long time during the growth of tumors.
Permanent Link: http://hdl.handle.net/11104/0187688