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Semisynthesis of C17:0 isoforms of sulphatide and glucosylceramide using immobilised sphingolipid ceramide N-deacylase for application in analytical mass spectrometry

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    0346664 - ÚMCH 2011 RIV GB eng J - Journal Article
    Kuchař, L. - Rotková, J. - Asfaw, B. - Lenfeld, Jiří - Horák, Daniel - Korecká, L. - Bílková, Z. - Ledvinová, J.
    Semisynthesis of C17:0 isoforms of sulphatide and glucosylceramide using immobilised sphingolipid ceramide N-deacylase for application in analytical mass spectrometry.
    Rapid Communications in Mass Spectrometry. Roč. 24, č. 16 (2010), s. 2393-2399. ISSN 0951-4198. E-ISSN 1097-0231
    R&D Projects: GA ČR GA203/09/0857
    Institutional research plan: CEZ:AV0Z40500505
    Keywords : reverse hydrolysis reaction * quantitative-determination * internal standards
    Subject RIV: CB - Analytical Chemistry, Separation
    Impact factor: 2.846, year: 2010

    Sphingolipid ceramide N-deacylase (SCDase, EC 3.5.1.69) is a hydrolytic enzyme isolated from Psendomonas sp. TK 4. In addition to its primary deacylation function, this enzyme is able to reacylate lyso-sphingolipids under specific conditions. We immobilized the enzyme on magnetic macroporous cellulose and used it to semisynthesise C17:0 glucosylceramide and C17:0 sulphatide.
    Permanent Link: http://hdl.handle.net/11104/0005994

     
     
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