Electrochemical Measurements on Supported Phospholipid Bilayers: Preparation, Properties and Ion Transport Using Incorporated Ionophores

0346606 - ÚFCH JH 2011 RIV DE eng J - Journal Article
Navrátil, Tomáš - Šestáková, Ivana - Štulík, Karel - Mareček, Vladimír
Electrochemical Measurements on Supported Phospholipid Bilayers: Preparation, Properties and Ion Transport Using Incorporated Ionophores.
Electroanalysis. Roč. 22, 17-18 (2010), s. 2043-2050. ISSN 1040-0397. E-ISSN 1521-4109.
[International Conference on Modern Electroanalytical Methods. Prague, 09.12.2009-14.12.2009]
R&D Projects: GA AV ČR IAA400400806
Institutional research plan: CEZ:AV0Z40400503
Keywords : voltammetry * phospholipid bilayers * Electrochemical impedance spectroscopy
Subject RIV: CG - Electrochemistry
Impact factor: 2.721, year: 2010

Model phospholipid bilayers (PLBs) were prepared from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) on a polycarbonate support and characterized by electrochemical impedance spectroscopy (EIS) measurements. The PLBs were formed by self-assembling in the 8.0 μm holes of the Isopore Membrane Filters, ionophores valinomycin and calcimycin (A23187) were incorporated. The effect of the applied voltage was monitored and reversible changes were observed. The ion transport across supported PLBs (SPLB) from DPPC was studied voltammetrically on the example of the cadmium ion and the ionophore calcimycin. Complex behavior was observed for cadmium ion transport with this ionophore in the presence of calcium ions.
Permanent Link: http://hdl.handle.net/11104/0187590