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Direct voltammetric analysis of DNA modified with enzymatically incorporated 7-deazapurines
- 1.0346582 - BFÚ 2011 RIV US eng J - Journal Article
Pivoňková, Hana - Horáková Brázdilová, Petra - Fojtová, Miloslava - Fojta, Miroslav
Direct voltammetric analysis of DNA modified with enzymatically incorporated 7-deazapurines.
Analytical Chemistry. Roč. 82, č. 16 (2010), s. 6807-6813. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA AV ČR(CZ) IAA400040901; GA MŠMT(CZ) LC06035
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : modified DNA * 7-deazapurines * voltammetric analysis
Subject RIV: BO - Biophysics
Impact factor: 5.874, year: 2010
Nucleic acids studies use 7-deazaguanine (G*) and 7-deazaadenine (A*) as analogues of natural purine bases. We show that enzymatic incorporation of G* can easily be monitored by simple ex situ voltammetric analysis of the modified DNA at carbon electrodes. G* is shown as an attractive electroactive marker for DNA, efficiently incorporable by PCR.
Permanent Link: http://hdl.handle.net/11104/0187576
Number of the records: 1