Antitumor activity od apoptotic nuclease TBN1 from L. esculentum

0346342 - BC 2011 RIV SK eng J - Journal Article
Matoušek, Jaroslav - Podzimek, Tomáš - Poučková, P. - Stehlík, Jan - Škvor, J. - Lipovová, P. - Matoušek, Josef
Antitumor activity od apoptotic nuclease TBN1 from L. esculentum.
Neoplasma. Roč. 57, č. 4 (2010), s. 339-348. ISSN 0028-2685. E-ISSN 1338-4317
R&D Projects: GA ČR GA521/06/1149; GA ČR GA521/09/1214
Institutional research plan: CEZ:AV0Z50510513; CEZ:AV0Z50450515
Keywords : anticancerogenic and antiproliferative nuclease * dsRNase * human solid malignant tumors
Subject RIV: FD - Oncology ; Hematology
Impact factor: 1.449, year: 2010

Nuclease from tomato (TBN1) wad produced by in planta biotechnology, purified and tested for its antitumor properties. The nuclease was cytostatic after its intratumoral administration to nude mice bearing human melanoma or prostate carcinoma, and also after tumor targeting by its intravenous administration as conjugate with PEG. Inhibitory effects of TBN1 on tumor growth were comparable to effects of bovine seminal RNase (BS-RNase), but the inhibition was reached at about ten times lower protein concentration. Intravenous administration of TBN1-PEG caused significant inhibition of tumor proliferation without obvious degenerative changes, while direct administration of TBN1 into melanoma tumors led to rapid tumor tissue degeneration. Simultaneously, TBN1 exhibited a lower degree of embryotoxicity compared to BS-RNase and other nucleases.
Permanent Link: http://hdl.handle.net/11104/0187396