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Conditions for succseful labeling of oxidation sensitive peptides by Na[125-I] - IODO-GEN(TM) system
- 1.0346291 - ÚOCHB 2011 RIV GB eng J - Journal Article
Elbert, Tomáš - Veselá, Iva
Conditions for succseful labeling of oxidation sensitive peptides by Na[125-I] - IODO-GEN(TM) system.
Journal of Labelled Compounds and Radiopharmaceuticals. Roč. 53, 5/6 (2010), s. 288-291. ISSN 0362-4803. E-ISSN 1099-1344.
[International Symposium on the Synthesis and Applications of Isotopes and Isotopically Labelled Compounds /10./. Chicago, 14.06.2009-18.06.2009]
R&D Projects: GA MŠMT LA 288
Institutional research plan: CEZ:AV0Z40550506
Keywords : Iodo-Gen * peptide * 125-I * radiolabelling
Subject RIV: CC - Organic Chemistry
Impact factor: 1.096, year: 2010
Very good and reproducible yields of 125-I-labeled peptides containing tryptophan residue were achieved when the radioidination reaction was performed with 1 to 3 mCi of carrrier free Na[125-I] in 1.5 ml eppendorf PP tubes precoated with 10 nmols of IODO-GEN(TM) in phosphate buffer saline (pH=7.8). Addition of bovine serum albumin to the solutions of radiolabeled peptides greatly reduced the losses during their workup.
Permanent Link: http://hdl.handle.net/11104/0187359
Number of the records: 1