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Analysis of wax ester molecular species by high performance liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry
- 1.0345947 - ÚOCHB 2011 RIV NL eng J - Journal Article
Vrkoslav, Vladimír - Urbanová, Klára - Cvačka, Josef
Analysis of wax ester molecular species by high performance liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry.
Journal of Chromatography A. Roč. 1217, č. 25 (2010), s. 4184-4194. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR GA203/09/0139
Institutional research plan: CEZ:AV0Z40550506
Keywords : Beeswax * Branched esters * Human hair * Jojoba * Mass spectrometry
Subject RIV: CC - Organic Chemistry
Impact factor: 4.194, year: 2010
High chromatographic resolution of wax esters (WEs) was achieved by non-aqueous reversed-phase liquid chromatography on a Nova-Pak C18 column by optimizing the acetonitrile/ethyl acetate mobile phase gradient. The retention behaviour of saturated, unsaturated and methyl-branched WEs was studied in this chromatographic system. The analytes were detected by atmospheric pressure chemical ionization mass spectrometry (APCI-MS). WEs provided simple full-scan spectra with abundant protonated molecules and low-intensity fragments. Collision-induced dissociation (CID) promoted identification of the WE molecular species. The responses of WEs were found to be dependent on the number of double bonds and on the alkyl-chain length. The HPLC/APCIMS was applied for the analysis of the WEs isolated from honeycomb beeswax, jojoba oil and human hair. Good agreement between reported results and the literature data was achieved, with several novel polyunsaturated WEs also being found.
Permanent Link: http://hdl.handle.net/11104/0187108
Number of the records: 1