First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic

0345901 - BC 2013 RIV US eng O - Others
Přibylová, Jaroslava - Špak, Josef - Kubelková, Darina - Petrzik, Karel
First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic.
Plant Disease. American Phytopathological Society. Roč. 94, č. 8 (2010), s. 1071-1071. ISSN 0191-2917. E-ISSN 1943-7692
R&D Projects: GA MŠMT(CZ) OC09022
Institutional research plan: CEZ:AV0Z50510513
Keywords : Virus * small fruits * pathogen detection
Subject RIV: EE - Microbiology, Virology

A collection of highbush blueberry (Vaccinium corymbosum L.) cultivars was surveyed in 2009. A total of 67 plants of 10 cultivars were observed for typical Blueberry red ringspot virus (BRRV) symptoms and were also assayed for BRRV infection using PCR. Extracted DNA from leaf tissue was used in PCR for BRRV detection with primer pair BRRV15/16. No BRRV symptoms were observed on the plants in early spring, yet BRRV was detected in one symptom-free bush of cv. Darrow by PCR. In July, typical symptoms developed on that and another cv. Darrow bush that was also positive by PCR. DNA fragments were amplified from total nucleic acid samples of both infected blueberry bushes using primers BRRV15/16. The amplicons were sequenced and revealed 97.5% nucleotide sequence identity to the BRRV (GenBank accession number AF404509). The 845 nucleotide sequence of the amplicon has been deposited at GenBank under accession number HM107773.
Permanent Link: http://hdl.handle.net/11104/0187073