Synthetic poly(amino acid) hydrogels with incorporated cell-adhesion peptides for tissue engineering

0345865 - ÚMCH 2011 RIV GB eng J - Journal Article
Studenovská, Hana - Vodička, Petr - Proks, Vladimír - Hlučilová, Jana - Motlík, Jan - Rypáček, František
Synthetic poly(amino acid) hydrogels with incorporated cell-adhesion peptides for tissue engineering.
Journal of Tissue Engineering and Regenerative Medicine. Roč. 4, č. 6 (2010), s. 454-463. ISSN 1932-6254. E-ISSN 1932-7005
R&D Projects: GA AV ČR KJB400500801; GA MŠMT 1M0538
Institutional research plan: CEZ:AV0Z40500505; CEZ:AV0Z50450515
Keywords : polyamino acid * hydrogel * porosity
Subject RIV: CD - Macromolecular Chemistry
Impact factor: 3.534, year: 2010

Preparation of soft poly(amino acid) hydrogels containing biomimetic cell-adhesive peptides was investigated. Covalently crosslinked gels were formed by radical co-polymerization of methacryloylated macromonomer poly[N5-(2-hydroxyethyl)-L-glutamine-stat-L-alanine-stat-methacryloyllysine] with 2-hydroxyethyl methacrylate (HEMA) as minor co-monomer. Hydrogels carrying biomimetic peptides were prepared by using methacryloylated peptides, such as methacryloyl-GGGRGDSG-OH and methacryloyl-GGGYIGSR-OH, as additional monomers in the polymerization mixture. While unmodified hydrogels showed very low cell adhesion, due to their highly hydrophilic nature, the incorporation of adhesive peptides significantly improved the adhesion and viability of seeded cells.
Permanent Link: http://hdl.handle.net/11104/0005960