PCR-DGGE Analysis of Bacterial Population Attached to the Bovine Rumen Wall

0345864 - ÚŽFG 2011 RIV CZ eng J - Journal Article
Lukáš, Filip - Šimůnek, Jiří - Mrázek, Jakub - Kopečný, Jan
PCR-DGGE Analysis of Bacterial Population Attached to the Bovine Rumen Wall.
Folia Microbiologica. Roč. 55, č. 4 (2010), s. 345-348. ISSN 0015-5632. E-ISSN 1874-9356
R&D Projects: GA ČR GA523/08/1091; GA ČR GA310/07/0414
Institutional research plan: CEZ:AV0Z50450515
Keywords : bacteria population * bovine rumen wall * feeding
Subject RIV: GG - Livestock Rearing
Impact factor: 0.977, year: 2010

We isolated and amplified by PCR 16S rDNA from bacteria attached to the bovine rumen wall and analyzed it by denaturing gradient gel electrophoresis (DGGE) with subsequent sequence analysis. The attached bacterial community differed from the bacteria of rumen content; however, no differences were observed among the five epithelial sampling sites taken from each animal. The DGGE profile of the bacterial population attached to the rumen wall represented a high inter-animal variation.
Permanent Link: http://hdl.handle.net/11104/0187047