Survival of Bifidobacteria Administered to Calves

0345856 - ÚŽFG 2011 RIV CZ eng J - Journal Article
Vlková, E. - Grmanová, M. - Killer, Jiří - Mrázek, Jakub - Kopečný, Jan - Bunešová, V. - Rada, V.
Survival of Bifidobacteria Administered to Calves.
Folia Microbiologica. Roč. 55, č. 4 (2010), s. 390-392. ISSN 0015-5632. E-ISSN 1874-9356
R&D Projects: GA ČR GA523/08/1091; GA ČR GD525/08/H060
Institutional research plan: CEZ:AV0Z50450515
Keywords : bifidobacteria * gastrointenistinal microflora * probiotics
Subject RIV: GM - Food Processing
Impact factor: 0.977, year: 2010

Twenty-five bifidobacteria were isolated from feces of calves. Isolates were identified, and their functional properties and antimicrobial activity were determined. From 10 strains with suitable properties rifampicin-resistant mutants (RRBs) were prepared and mixture of RRBs was administered to 2-d-old calves. These strains were identified by sequencing as Bifidobacterium animalis ssp. animalis (6 strains), B. thermophilum (2 strains), B. choerinum (1 strain) and B. longum ssp. suis (1 strain). The control group was without probiotic treatment. Survival ability of administered bifidobacteria was monitored in fecal samples by cultivation on modified TPY agar supplemented with mupirocin, acetic acid, and rifampicin. Administered bifidobacteria survived in gastrointestinal tract of calves for at least 60 d. Other bacteria were also determined after cultivation using fluorescence in situ hybridization (FISH). Bifidobacteria and lactobacilli dominated in fecal microflora.
Permanent Link: http://hdl.handle.net/11104/0187040