Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy

0345815 - ÚFCH JH 2011 RIV NL eng J - Journal Article
Macháň, Radek - Hof, Martin
Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy.
Biochimica Et Biophysica Acta-Biomembranes. Roč. 1798, č. 7 (2010), s. 1377-1391. ISSN 0005-2736. E-ISSN 1879-2642
R&D Projects: GA ČR GA203/08/0114; GA AV ČR GEMEM/09/E006
Institutional research plan: CEZ:AV0Z40400503
Keywords : supported lipid bilayer * giant unilamellar vesicle * fluorescence recovery after photobleaching
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 4.647, year: 2010

Investigation of lipid lateral mobility in biological membranes and their artificial models provides information on membrane dynamics and structure: methods based on optical microscopy are very convenient for such investigations. We focus on fluorescence correlation spectroscopy (FCS), explain its principles and review its state of the art versions such as 2-focus. Z-scan or scanning FCS, which overcome most artefacts of standard FCS (especially those resulting from the need for an external calibration) making it a reliable and versatile method. FCS is also compared to single particle tracking and fluorescence photobleaching recovery and the applicability and the limitations of the methods are briefly reviewed. We discuss several key questions of lateral mobility investigation in planar lipid membranes, namely the influence which membrane and aqueous phase composition (ionic strength and sugar content), choice of a fluorescent tracer molecule, ...
Permanent Link: http://hdl.handle.net/11104/0187002