A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae

0345684 - BC 2011 RIV NL eng J - Journal Article
Lenz, Ondřej - Beran, Pavel - Fousek, Jan - Mráz, Ivan
A microarray for screening the variability of 16S–23S rRNA internal transcribed spacer in Pseudomonas syringae.
Journal of Microbiological Methods. Roč. 82, č. 1 (2010), s. 90-94. ISSN 0167-7012. E-ISSN 1872-8359
R&D Projects: GA ČR GP522/07/P338
Institutional research plan: CEZ:AV0Z50510513
Keywords : microarray * ITS1 * mosaicism
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.018, year: 2010

The 16S–23S ribosomal internal transcribed spacer (ITS1) is often used as a subspecies or strain-specific molecular marker for various kinds of bacteria. However, the presence of different copies of ITS1 within a single genome has been reported. Such mosaicism may influence correct typing of many bacteria and therefore knowledge about exact configuration of this region in a particular genome is essential. In order to screen the variability of ITS1 among and within Pseudomonas syringae genomes, an oligonucleotide microarray targeting different configurations of ITS1 was developed. The microarray revealed seven distinct variants in 13 pathovars tested and detected mosaicism within the genomes of P. syringae pv. coronafaciens, pisi, syringae and tabaci. In addition, the findings presented here challenge the using of rRNA analysis for pathovar and strain determination.
Permanent Link: http://hdl.handle.net/11104/0186895