Arsenicals produce stable progressive changes in DNA methylation patterns that are linked to malignant transformation of immortalized urothelial cells

0345683 - BC 2011 RIV US eng J - Journal Article
Jensen, T.J. - Novák, Petr - Wnek, S.M. - Gandolfi, A.J. - Futscher, B. W.
Arsenicals produce stable progressive changes in DNA methylation patterns that are linked to malignant transformation of immortalized urothelial cells.
Toxicology and Applied Pharmacology. Roč. 241, č. 2 (2009), s. 221-229. ISSN 0041-008X. E-ISSN 1096-0333
Institutional research plan: CEZ:AV0Z50510513
Keywords : DNA methylation * Epigenetic * Arsenic
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.359, year: 2009

Aberrant DNA methylation participates in carcinogenesis and is a molecular hallmark of a tumor cell. We used a model of arsenical-induced malignant transformation of immortalized human urothelial cells and DNA methylation microarrays to examine the extent and temporal nature of changes in DNA methylation that occur during the transition from immortal to malignantly transformed. Our data presented herein suggest that during arsenical-induced malignant transformation, aberrant DNA methylation occurs non-randomly, progresses gradually at hundreds of gene promoters, and alters expression of the associated gene, and these changes are coincident with the acquisition of malignant properties, such as anchorage independent growth and tumor formation in immunocompromised mice.
Permanent Link: http://hdl.handle.net/11104/0186894