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New applications of pHluorin - measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis
- 1.0345607 - FGÚ 2011 RIV GB eng J - Journal Article
Marešová, Lydie - Hošková, B. - Urbánková, E. - Chaloupka, R. - Sychrová, Hana
New applications of pHluorin - measuring intracellular pH of prototrophic yeasts and determining changes in the buffering capacity of strains with affected potassium homeostasis.
Yeast. Roč. 27, č. 6 (2010), s. 317-325. ISSN 0749-503X. E-ISSN 1097-0061
R&D Projects: GA MŠMT(CZ) LC531; GA AV ČR(CZ) KJB500110701
Institutional research plan: CEZ:AV0Z50110509
Keywords : pH maintenance * Saccharomyces cerevisiae * fluorescence measurements
Subject RIV: ED - Physiology
Impact factor: 1.626, year: 2010
We constructed a new pHluorin plasmid with the dominant selection marker (KanMX). This plasmid allows pH measurements in cells without auxotrophic mutations and/or grown in chemically indefinite media. We also utilised pHluorin in a new method of measuring the buffering capacity of yeast cell cytosol in vivo
Permanent Link: http://hdl.handle.net/11104/0186841
Number of the records: 1