Surface plasmon resonance imaging for parallelized detection of protein biomarkers

0345547 - ÚFE 2012 RIV US eng C - Conference Paper (international conference)
Piliarik, Marek - Párová, Lucie - Vaisocherová, Hana - Homola, Jiří
Surface plasmon resonance imaging for parallelized detection of protein biomarkers.
Optical Sensors 2009. Vol. 7356. Bellingham, Washington: SPIE, 2009 - (Baldini, F.; Homola, J.; Lieberman, R.), 73560D1-73560D8. Proceedings of SPIE, 7356. ISBN 9780819476302. ISSN 0277-786X.
[Optical Sensors 2009. Praha (CZ), 20.04.2009-22.04.2009]
R&D Projects: GA AV ČR KAN200670701
Institutional research plan: CEZ:AV0Z20670512
Keywords : Surface plasmon resonance imaging * Biosensor * Protein detection
Subject RIV: JB - Sensors, Measurment, Regulation

We report a novel high-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of protein biomarkers. The biosensor is based on a high-performance SPR imaging sensor with polarization contrast and internal referencing which yields a considerably higher sensitivity and resolution than conventional SPR imaging systems (refractive index resolution 2 × 10-7 RIU). We combined the SPR imaging biosensor with microspotting to create an array of antibodies. DNA-directed protein immobilization was utilized for the spatially resolved attachment of antibodies. Using Human Chorionic Gonadotropin (hCG) as model protein biomarker, we demonstrated the potential for simultaneous detection of proteins in up to 100 channels.
Permanent Link: http://hdl.handle.net/11104/0186795