The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors

0345026 - ÚEM 2011 RIV NL eng J - Journal Article
Kubinová, Šárka - Horák, Daniel - Kozubenko, Nataliya - Vaněček, Václav - Proks, Vladimír - Price, J. - Cocks, G. - Syková, Eva
The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors.
Biomaterials. Roč. 31, č. 23 (2010), s. 5966-5975. ISSN 0142-9612. E-ISSN 1878-5905
R&D Projects: GA MŠMT(CZ) LC554; GA AV ČR(CZ) IAA500390902
Grant - others:GA MŠk(CZ) 1M0538; GA ČR(CZ) GD309/08/H079; GA ČR(CZ) KAN200200651; GA ČR(CZ) GAP108/10/1560; EC FP6 project ARTEMIS(XE) LSHM-CT-2007-037862
Program: 1M; KA; GA
Institutional research plan: CEZ:AV0Z50390703; CEZ:AV0Z40500505
Keywords : cell adhesion * laminin * mesenchymal stem cell
Subject RIV: FH - Neurology
Impact factor: 7.883, year: 2010
http://arl-repository.lib.cas.cz/uloziste_av/UEM-P/cav_un_epca-0345026_01.pdf

Modifications of poly(2-hydroxyethyl methacrylate) (PHEMA) with laminin-derived Ac-CGGASIKVAVS-OH peptide sequences have been developed to construct scaffolds that promote cell adhesion and neural differentiation. The Ac-CGGASIKVAVS-OH peptide was immobilized to the sulfhydryl groups by 2,2'-dithiodipyridine linking reagent via 2-[4-(2-pyridyldisulfanyl)butanamido]ethyl methacrylate. Superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds significantly increased the number of attached mesenchymal stem cells and their growth area on the hydrogel surface in the absence and in the presence of serum in the culture medium. Additionally, the Ac-CGGASIKVAVS-OH peptide supported the attachment, proliferation, differentiation and process spreading of human fetal neural stem cells during the first two weeks of expansion and contributed to the formation of a high percentage of more mature neural cells after four weeks of expansion.
Permanent Link: http://hdl.handle.net/11104/0005938