Surface modification of cyclic olefin copolymers for osteochondral defect repair can increase pro-destructive potential of human chondrocytes in vitro

0343392 - ÚFCH JH 2011 RIV CZ eng J - Journal Article
Polanská, M. - Hulejová, H. - Petrtýl, M. - Bastl, Zdeněk - Spirovová, Ilona - Kruliš, Zdeněk - Horák, Zdeněk - Veigl, D. - Šenolt, L.
Surface modification of cyclic olefin copolymers for osteochondral defect repair can increase pro-destructive potential of human chondrocytes in vitro.
Physiological Research. Roč. 59, č. 2 (2010), s. 247-253. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA ČR GA106/06/0761
Institutional research plan: CEZ:AV0Z40400503; CEZ:AV0Z40500505
Keywords : osteochondral defects * cycloolefin copolymer * chondrocytes * biocompatibility
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 1.646, year: 2010

Materials on the basis of cycloolefin copolymers (COC) are suitable for subchondral defect repairs. The objective of this study was to evaluate the influence of surface modification of COC and COC/LLDPE blends on the viability and gene expression of chondrocytes. Human chondrocytes were incubated on the surface of the studied materials. Half of the materials were plasmatically modified with a subsequent type II collagen application. The gene expression of matrix metalloproteinases (MMP-1,-3,-13), pro-inflammatory cytokines (IL-1, TNF-alpha) and apoptotic molecules (BAX, Bcl-2) was evaluated using quantitative Taq-Man PCR after 48 h incubation. Chondrocyte viability was evaluated by the MTT test after 2, 4 and 8 days of incubation. The synthesis of MMPs was measured by ELISA assay in cell culture medium after 48 h of incubation.
Permanent Link: http://hdl.handle.net/11104/0185883