A Review on the Effects of Supercritical Carbon Dioxide on Enzyme Activity

0343315 - ÚEB 2011 RIV US eng J - Journal Article
Wimmer, Zdeněk - Zarevúcka, Marie
A Review on the Effects of Supercritical Carbon Dioxide on Enzyme Activity.
International Journal of Molecular Sciences. Roč. 11, č. 1 (2010), s. 233-253. E-ISSN 1422-0067
R&D Projects: GA MŠMT 2B06024
Institutional research plan: CEZ:AV0Z50380511; CEZ:AV0Z40550506
Keywords : enzyme * supercritical carbon dioxide * synthesis
Subject RIV: CC - Organic Chemistry
Impact factor: 2.279, year: 2010

Different types of enzymes such as lipases, several phosphatases, dehydrogenases, oxidases, amylases and others are well suited for the reactions in SC-CO2. The stability and the activity of enzymes exposed to carbon dioxide under high pressure depend on enzyme species, water content in the solution and on the pressure and temperature of the reaction system. The three-dimensional structure of enzymes may be significantly altered under extreme conditions, causing their denaturation and consequent loss of activity. If the conditions are less adverse, the protein structure may be largely retained. Minor structural changes may induce an alternative active protein state with altered enzyme activity, specificity and stability.
Permanent Link: http://hdl.handle.net/11104/0185821