Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope

0343182 - ÚVGZ 2011 RIV GB eng J - Journal Article
Reshak, Ali H - Sarafis, V. - Heintzmann, R.
Second harmonic imaging of chloroplasts using the two-photon laser scanning microscope.
Micron. Roč. 40, č. 3 (2009), s. 378-385. ISSN 0968-4328. E-ISSN 1878-4291
Institutional research plan: CEZ:AV0Z60870520
Keywords : 2PLSM * collagen * generatin * scattering * membrane * fibrils
Subject RIV: BO - Biophysics
Impact factor: 1.626, year: 2009

Background: Second harmonic information reveals information about the structure of spatially oriented structures with an asymmetry. We study the second harmonic images of the grana and intergranal parts of chloroplasts in the leaves of the shade moss Plagiomnium affine. Results: A two-photon microscope with blocking filters to suppress fluorescence generated both forward and backward second harmonic illumination to be captured. In the starch free chloroplasts of the single cell layered laminae strong second harmonic generation (SHG) from the granal regions was emitted. Upon illumination the chloroplasts changed their orientation affecting the SHG signal. Chloroplast is reoriented upon illumination.
Permanent Link: http://hdl.handle.net/11104/0185716