Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei

0342948 - BC 2011 RIV DE eng J - Journal Article
Verner, Zdeněk - Paris, Zdeněk - Lukeš, Julius
Mitochondrial membrane potential-based genome-wide RNAi screen of Trypanosoma brucei.
Parasitology Research. Roč. 106, č. 5 (2010), s. 1241-1244. ISSN 0932-0113. E-ISSN 1432-1955
Institutional research plan: CEZ:AV0Z60220518
Keywords : GENE-FUNCTION * INTERFERENCE * mitochondrion * SUBUNITS
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.812, year: 2010

We have screened the Trypanosoma brucei genome-wide RNAi library by staining the procyclics with the dye JC-1 followed by sorting the differentially stained cells by flow cytometry. This allowed us to highly enrich for cells in which mitochondrial membrane potential was decreased. We have further validated a subset of selected clones by a reverse approach in which we showed that cloning the selected genomic regions into another RNAi vector also results in a drop in mitochondrial membrane potential.
Permanent Link: http://hdl.handle.net/11104/0185549