Second harmonic generation from thick leaves using the two-photon laser scanning microscope

0342935 - ÚVGZ 2011 RIV GB eng J - Journal Article
Reshak, Ali H
Second harmonic generation from thick leaves using the two-photon laser scanning microscope.
Micron. Roč. 4, č. 40 (2009), s. 455-462. ISSN 0968-4328. E-ISSN 1878-4291
Institutional research plan: CEZ:AV0Z60870520
Keywords : Duckweed Lemna minuta
Subject RIV: BO - Biophysics
Impact factor: 1.626, year: 2009

A laser-scanning microscope using Second harmonic generation (SHG) as a probe is shown to produce high-resolution images of duckweed Lemna minuta leaves. These leaves are multi-cell layer thick. Second harmonic light is generated by a tightly focused short pulse laser beam and is collected by two objectives yielding forward and backward second harmonic digital images. This measurement shows that the signal of the second harmonic imaging in the forward and backward directions depends on the thickness of the chloroplast and that the forward-SH image was brighter than the backward-SH image. The image intensity also depended on the orientation of the chloroplast in relation to the illuminating polarization direction. Their light-induced re-orientation which was affected by the intensity of the illumination could be observed during the experiments.
Permanent Link: http://hdl.handle.net/11104/0185537