Activation of protective cell-mediated immune response in gastric mucosa during Cryptosporidium muris infection and re-infection in immunocompetent mice

0342901 - BC 2011 RIV DE eng J - Journal Article
Jalovecká, M. - Sak, Bohumil - Kváč, Martin - Květoňová, Dana - Kučerová, Z. - Salát, Jiří
Activation of protective cell-mediated immune response in gastric mucosa during Cryptosporidium muris infection and re-infection in immunocompetent mice.
Parasitology Research. Roč. 106, č. 5 (2010), s. 1159-1166. ISSN 0932-0113. E-ISSN 1432-1955
R&D Projects: GA AV ČR KJB500960701
Institutional research plan: CEZ:AV0Z60220518
Keywords : Cryptosporidium muris * T-lymphocyte migration * gastric mucosa
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 1.812, year: 2010

The immune response in the stomach mucosa during the primary infection and re-infection with Cryptosporidium muris (TS03 and CB03) in immunocompetent BALB/c mice was characterized using flow cytometry analysis and measurement of IFN-gamma and IL10 by enzyme-linked immunosorbent assays (ELISA). Significantly, elevated migration of T lymphocytes (more than 1,000-fold), especially CD8+ T lymphocytes, to the stomach mucosa occurred during primary infection and persisted for more than 2 months after its resolution. The ex vivo cultures of splenocytes revealed very low levels of IFN-gamma production during the course of the primary infection (0.5 ng/ml), whereas in the following re-exposure to the parasites, the concentration of IFN-gamma rapidly increased 22-fold. These results imply that the CD8+ T lymphocytes are involved in the immune response to gastric cryptosporidiosis and could play an important role in the elimination of C. muris infection in mice.
Permanent Link: http://hdl.handle.net/11104/0185510