Filamoeba sp. Isolated from Hot-water Piping System, a Host of Legionella Closely Related to Legionella micdadei

0342895 - BC 2011 RIV PL eng J - Journal Article
Dyková, Iva - Hrubá, L. - Kostka, Martin - Pecková, Hana
Filamoeba sp. Isolated from Hot-water Piping System, a Host of Legionella Closely Related to Legionella micdadei.
Acta Protozoologica. Roč. 48, č. 4 (2009), s. 321-327. ISSN 0065-1583. E-ISSN 1689-0027
R&D Projects: GA ČR GA524/09/0137; GA MŠMT LC522
Institutional research plan: CEZ:AV0Z60220518
Keywords : Filamoeba sp. * intracellular * Legionella sp.
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 0.775, year: 2009

An amoeba was isolated from 56 ºC hot water, sampled in a piping system that was monitored for the presence of legionellae. The amoeba strain JIH56 was derived from the primary isolate by subculturing at 38ºC. It was assigned to the genus Filamoeba Page, 1967 using light microscopical, ultrastructural and molecular criteria. In the cytoplasm of Filamoeba trophozoites were found Legionella-like bacteria. These were determined using Legionella-specific PCR assay followed by sequencing. Phylogenetic analysis and a similarity search revealed a close relationship of the newly determined legionella sequence with sequences of Legionella micdadei (syn. Tatlockia micdadei), the Pittsburgh pneumonia agent. Phylogenetic analysis of the Filamoeba sequences available to date suggests that the ATCC 50430 strain was misidentified.
Permanent Link: http://hdl.handle.net/11104/0185504