Nucleotide variability of Ťahyňa virus (Bunyaviridae, Orthobunyavirus) small (S) and medium (M) genomic segments in field strains differing in biological properties

0342878 - BC 2011 RIV NL eng J - Journal Article
Kilian, P. - Růžek, Daniel - Danielová, V. - Hypša, Václav - Grubhoffer, Libor
Nucleotide variability of Ťahyňa virus (Bunyaviridae, Orthobunyavirus) small (S) and medium (M) genomic segments in field strains differing in biological properties.
Virus Research. Roč. 149, č. 1 (2010), s. 119-123. ISSN 0168-1702. E-ISSN 1872-7492
R&D Projects: GA MŠMT(CZ) LC06009
Institutional research plan: CEZ:AV0Z60220518
Keywords : Tahyna virus * Bunyavirus * california group * genetic variability * virulence
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.905, year: 2010

Tahyna virus (TAHV), a mosquito-borne bunyavirus (California group), is frequently associated with inapparent or influenza-like (Valtice fever) infections in humans. Field TAHV strains exhibit a high variability in their biological properties with respect to virulence for laboratory mouse, temperature-sensitivity or character of plaques in cell culture. We analyzed complete nucleotide sequences of the small (S) and medium (M) genomic segments of field TAHV strains with different combinations of phenotypic markers. S segment was highly conservative in all analyzed TAHV strains. Within the M segment, the highest variability was observed in the G(C) gene encoding viral envelope protein and to a less extent also in the NSm gene. However, 5' and 3' non-coding regions of M segment, as well as in G(N) gene exhibited highly conservative pattern, indicating its functional importance, but minor or no role in the determination of biological properties of TAHV field strains.
Permanent Link: http://hdl.handle.net/11104/0185490