Characterization of ferritin 2 for the control of tick infestations

0342866 - BC 2012 RIV GB eng J - Journal Article
Hajdušek, Ondřej - Almazán, C. - Loosová, Gabriela - Villar, M. - Canales, M. - Grubhoffer, L. - Kopáček, Petr - de la Fuente, J.
Characterization of ferritin 2 for the control of tick infestations.
Vaccine. Roč. 28, č. 17 (2010), s. 2993-2998. ISSN 0264-410X. E-ISSN 1873-2518
R&D Projects: GA AV ČR IAA600960910; GA MŠMT(CZ) LC06009
Institutional research plan: CEZ:AV0Z60220518
Keywords : tick ferritin 2 * tick-protective vaccine
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.572, year: 2010

Tick iron metabolism pathway was targeted in an effort to identify new tick protective antigens. Recombinant Ixodes ricinus (IrFER2) and Rhipicephalus microplus (RmFER2) ferritin 2 proteins were expressed in Escherichia coli and used to immunize rabbits and cattle, respectively. Vaccination with IrFER2 reduced I. ricinus tick numbers, weight and fertility in rabbits with an overall vaccine efficacy (E) of 98%. Control of cattle tick, R. microplus and Rhipicephalus annulatus infestations was obtained in vaccinated cattle with overall E of 64% and 72%, respectively. Notably, the efficacy of the RmFER2 vaccine was similar to that obtained with Bm86 against R. microplus. These collective results demonstrated the feasibility of using ferritin 2 to develop vaccines for the control of tick infestations.
Permanent Link: http://hdl.handle.net/11104/0185480