Number of the records: 1
Phosphomannose isomerase gene for selection in lettuce (Lactuca sativa L.) transformation
- 1.0342588 - BC 2011 RIV PL eng J - Journal Article
Bříza, Jindřich - Růžičková, N. - Niedermeierová, Hana - Dusbábková, Jana - Vlasák, Josef
Phosphomannose isomerase gene for selection in lettuce (Lactuca sativa L.) transformation.
Acta Biochimica Polonica. Roč. 57, č. 1 (2010), s. 63-68. ISSN 0001-527X. E-ISSN 1734-154X
Institutional research plan: CEZ:AV0Z50510513
Keywords : pmi * npt II * Lactuca sativa
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.234, year: 2010
A positive selection system using phosphomannose isomerase was employed for Agrobacterium tumefaciens mediated transformation of lettuce (Lactuca sativa L. var. ‘Achát’). It was shown that the mannose-based selection system works very well with the lettuce genotype used, reaching up to 25 % transformation efficiency on the medium with 20 g/L mannose and 20 g/L sucrose.
Permanent Link: http://hdl.handle.net/11104/0185286
Number of the records: 1