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Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines
- 1.0342374 - ÚOCHB 2011 RIV DE eng J - Journal Article
Raindlová, Veronika - Pohl, Radek - Šanda, Miloslav - Hocek, Michal
Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines.
Angewandte Chemie - International Edition. Roč. 49, č. 6 (2010), s. 1064-1066. ISSN 1433-7851. E-ISSN 1521-3773
R&D Projects: GA MŠMT LC512; GA ČR GA203/09/0317
Institutional research plan: CEZ:AV0Z40550506
Keywords : aldehydes * nucleotides * nucleoside triphosphates * DNA polymerase * DNA conjugates
Subject RIV: CC - Organic Chemistry
Impact factor: 12.730, year: 2010
Reactive aldehyde-modified DNA was prapered in two steps by cross-coupling reactions of halogenated dNTPs with 4-formylthiophene-2-boronic acid followed by polymerase (PEX or PCR) incorporations of the modified nucleotides to DNA. Aqueous hydrazone-formation was used in conjugation with aryl hydrazines for DNA staining.
Permanent Link: http://hdl.handle.net/11104/0185124
Number of the records: 1