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Synthesis of nucleoside and nucleotide conjugates of bile acids, and polymerase construction of bile acid-functionalized DNA
- 1.0342359 - ÚOCHB 2011 RIV GB eng J - Journal Article
Ikonen, Satu - Macíčková-Cahová, Hana - Pohl, Radek - Šanda, Miloslav - Hocek, Michal
Synthesis of nucleoside and nucleotide conjugates of bile acids, and polymerase construction of bile acid-functionalized DNA.
Organic & Biomolecular Chemistry. Roč. 8, č. 5 (2010), s. 1194-1201. ISSN 1477-0520. E-ISSN 1477-0539
R&D Projects: GA MŠMT LC512; GA ČR GA203/09/0317
Institutional research plan: CEZ:AV0Z40550506
Keywords : steroids * nucleosides * nucleoside triphosphates * DNA polymerase
Subject RIV: CC - Organic Chemistry
Impact factor: 3.451, year: 2010
Sonogashira cross-coupling reactions of 5-iodopyrimidine or 7-iodo-7-deazaadenine nucleosides with bile acid-derived terminal acetylenes gave the corresponding bile acid-nucleoside conjugates. Analogous reactions of halogenated nucleoside triphosphates gave directly bile acid-modified dNTPs. Enzymatic incorporation of these modified nucleotides to DNA was successfully performed using Phusion polymerase for primer extension.
Permanent Link: http://hdl.handle.net/11104/0185111
Number of the records: 1