Protein alterations induced by long-term agonist treatment of HEK293 cells expressing thyrotropin-releasing hormone receptor and G11alpha protein

0342117 - FGÚ 2011 RIV US eng J - Journal Article
Drastichová, Z. - Bouřová, Lenka - Hejnová, L. - Jedelský, P. - Svoboda, Petr - Novotný, J.
Protein alterations induced by long-term agonist treatment of HEK293 cells expressing thyrotropin-releasing hormone receptor and G11alpha protein.
Journal of Cellular Biochemistry. Roč. 109, č. 1 (2010), s. 255-264. ISSN 0730-2312. E-ISSN 1097-4644
R&D Projects: GA MŠMT(CZ) LC554; GA ČR(CZ) GA309/06/0121; GA ČR(CZ) GD305/08/H037
Institutional research plan: CEZ:AV0Z50110509
Keywords : Thyrotropin-releasing hormone * Gq/11 protein * proteomics
Subject RIV: ED - Physiology
Impact factor: 3.122, year: 2010

This study aimed to determine protein alterations induced by thyrotropin-releasing hormone in plasma membrane-enriched fraction of HEK293, which was prepared by Percoll gradient centrifugation. The high enrichment in plasma membrane proteins of this preparation was confirmed by a multifold increase in the number of TRH receptors and agonist stimulated G-protein activity, compared to postnuclear supernatant. Using 2D-electrophoresis we were able to determine 61 altered proteins in this fraction isolated from cells treated with TRH (1x10-5 M, 16 h), some of which belong to antiapoptotic proteins and their increased amount after TRH treatment were in line with the presumed antiapoptotic effect of TRH
Permanent Link: http://hdl.handle.net/11104/0184938