Temperature dependence of N-methyl-d-aspartate receptor channels and N-methyl-d-aspartate receptor excitatory postsynaptic currents

0342116 - FGÚ 2011 RIV GB eng J - Journal Article
Kořínek, Miroslav - Sedláček, Miloslav - Cais, Ondřej - Dittert, Ivan - Vyklický ml., Ladislav
Temperature dependence of N-methyl-d-aspartate receptor channels and N-methyl-d-aspartate receptor excitatory postsynaptic currents.
Neuroscience. Roč. 165, č. 3 (2010), s. 736-748. ISSN 0306-4522. E-ISSN 1873-7544
R&D Projects: GA MŠMT(CZ) 1M0517; GA MŠMT(CZ) LC554; GA ČR(CZ) GA309/07/0271
Grant - others:EC(XE) LSHM-CT-2007-037765
Institutional research plan: CEZ:AV0Z50110509
Keywords : hypothermia * patch-clamp recording
Subject RIV: ED - Physiology
Impact factor: 3.215, year: 2010

The article focuses on the problematic of temperature dependence of deactivation of native N-Methyl-D-Aspartate (NMDA) receptors in neuronal cultures and the temperature dependence of excitatory postsynaptic currents mediated by synaptic NMDA receptors in rat cortical slices. Our electrophysiology experiments found that both native as well as synaptic receptors exhibit significantly weaker temperature dependence compared to recombinant GluN1/GluN2B NMDA receptors. Synaptic NMDA receptors have characteristics of receptors with low desensitization (20%). Double exponential time course of NMDA receptor deactivation originates mainly from dissociation of agonist and desensitization
Permanent Link: http://hdl.handle.net/11104/0184937