Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris'

0342115 - BC 2011 RIV DE eng J - Journal Article
Fránová, Jana - Petrzik, Karel
Asparagus officinalis: A New Host of 'Candidatus Phytoplasma asteris'.
Journal of Phytopathology - Phytopathologische Zeitschrift. Roč. 158, č. 4 (2010), s. 317-320. ISSN 0931-1785. E-ISSN 1439-0434
R&D Projects: GA AV ČR(CZ) 1QS500510558
Institutional research plan: CEZ:AV0Z50510513
Keywords : 16S Ribosomal-RNA * mycoplasmalike organisms * gene-sequences
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.937, year: 2010

Asparagus officinalis plants with severe fasciation of some spears were observed in southern Bohemia between 1998 and 2007. Nucleic acids extracted from these and asymptomatic plants were assayed with nested polymerase chain reaction (PCR) using the phytoplasma-specific universal ribosomal primers P1/P7 and R16F2n/R2. The restriction profiles obtained from digestion of the PCR products with five endonucleases (AluI, HhaI, KpnI, MseI and RsaI) were identical in all phytoplasmas infecting asparagus in the Czech Republic and indistinguishable from those of phytoplasmas in the aster yellows group (subgroup 16SrI-B). Sequence analysis of 1754 bp of the ribosomal operon indicated that the closest related phytoplasmas were those associated with epilobium phyllody and onion yellows. This is the first report of the natural occurrence of 'Candidatus Phytoplasma asteris' in A. officinalis.
Permanent Link: http://hdl.handle.net/11104/0184936