The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod

0341849 - FGÚ 2011 RIV US eng J - Journal Article
Čebašek, V. - Eržen, I. - Vyhnal, Aleš - Janáček, Jiří - Ribarič, S. - Kubínová, Lucie
The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod.
Microvascular Research. Roč. 79, č. 1 (2010), s. 40-46. ISSN 0026-2862. E-ISSN 1095-9319
R&D Projects: GA MŠMT(CZ) MEB090910; GA MŠMT(CZ) LC06063; GA ČR(CZ) GD204/09/H084
Institutional research plan: CEZ:AV0Z50110509
Keywords : confocal microscopy * capillaries * length measurement
Subject RIV: EA - Cell Biology
Impact factor: 2.390, year: 2010

We compared two methods of evaluation of capillary supply of individual skeletal muscle fibers, the traditional one using 2D images of thin transverse sections by the number of capillary profiles around a fiber (CAF) and the 3D approach where the mean length of capillaries around individual muscle fibers per fiber length (Lcap/Lfib) is measured from 3D images acquired by confocal microscopy. In models we showed that Lcap/Lfib was sensitive to different arrangements of capillaries, while CAF underestimated capillarization since it could not detect the increased length of capillary bed. In true muscle samples, we detected statistically significant differences in the capillary supply of control and denervated rat soleus muscles by both 2D and 3D methods. Lcap/Lfib was larger than CAF in control muscles reflecting their more complicated capillary bed
Permanent Link: http://hdl.handle.net/11104/0184710