Proteome Mining of Human Follicular Fluid Reveals a Crucial Role of Complement Cascade and Key Biological Pathways in Women Undergoing in Vitro Fertilization

0341837 - ÚŽFG 2011 RIV US eng J - Journal Article
Jarkovská, Karla - Martinková, Jiřina - Lišková, Lucie - Halada, Petr - Moos, J. - Rezábek, K. - Gadher, S. J. - Kovářová, Hana
Proteome Mining of Human Follicular Fluid Reveals a Crucial Role of Complement Cascade and Key Biological Pathways in Women Undergoing in Vitro Fertilization.
Journal of Proteome Research. Roč. 9, č. 3 (2010), s. 1289-1301. ISSN 1535-3893. E-ISSN 1535-3907
R&D Projects: GA MZd(CZ) NS9781; GA AV ČR 1QS500450568
Institutional research plan: CEZ:AV0Z50450515; CEZ:AV0Z50200510
Keywords : Human follicular fluid * plasma * assisted reproduction
Subject RIV: FK - Gynaecology, Childbirth
Impact factor: 5.460, year: 2010

The primary aim of this study was to search for specific differences in protein composition of human follicular fluid (HFF) and plasma in order to identify proteins that accumulate or are absent in HFF. Depletion of abundant proteins combined with multidimensional protein fractionation allowed the study of middle- and lower-abundance proteins. Paired comparison study examining HFF with plasma/serum from women undergoing successful IVF revealed important differences in the protein composition which may improve our knowledge of the follicular microenvironment and its biological role. This study showed involvement of innate immune function of complement cascade in HFF Complement inhibition and the presence of C-terminal fragment of perlecan suggested possible links to angiogenesis which is a vital process in folliculogenesis and placental development. Differences in proteins associated with blood coagulation were also found in the follicular milieu.
Permanent Link: http://hdl.handle.net/11104/0184701