Modulation of AMPA excitatory postsynaptic currents in the spinal cord dorsal horn neurons by insulin

0341812 - FGÚ 2011 RIV GB eng J - Journal Article
Špicarová, Diana - Paleček, Jiří
Modulation of AMPA excitatory postsynaptic currents in the spinal cord dorsal horn neurons by insulin.
Neuroscience. Roč. 166, č. 1 (2010), s. 305-311. ISSN 0306-4522. E-ISSN 1873-7544
R&D Projects: GA ČR(CZ) GA305/06/1115; GA ČR GA305/09/1228; GA MŠMT(CZ) LC554
Institutional research plan: CEZ:AV0Z50110509
Keywords : pain * synaptic modulation * insulin
Subject RIV: FH - Neurology
Impact factor: 3.215, year: 2010

Glutamate AMPA receptors are critical for sensory transmission at the spinal cord dorsal horn (DH). Plasma membrane AMPA receptor endocytosis that can be induced by insulin may underlie long term modulation of synaptic transmission. In this work the effect of insulin application on fast excitatory postsynaptic currents (EPSCs) mediated by AMPA receptors evoked in DH neurons was evaluated. Acute spinal cord slices from 6 to 10 day old mice were used to record EPSCs evoked in visually identified superficial DH neurons by dorsal root primary afferent stimulation. AMPA EPSCs could be evoked in all of the tested neurons. In 75% of the neurons the size of the AMPA EPSCs was reduced to 62.1% and to 68.9% of the control values when 0.5 or 10 _M insulin was applied.Our results suggest a possible role of the insulin pathway in modulation of sensory and nociceptive synaptic transmission in the spinal cord.
Permanent Link: http://hdl.handle.net/11104/0184685