Locust adipokinetic hormone mobilizes diacylglycerols selectively

0341363 - BC 2011 RIV US eng J - Journal Article
Tomčala, Aleš - Bártů, Iva - Šimek, Petr - Kodrík, Dalibor
Locust adipokinetic hormone mobilizes diacylglycerols selectively.
Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology. Roč. 156, č. 1 (2010), s. 26-32. ISSN 1096-4959. E-ISSN 1879-1107
R&D Projects: GA ČR GA522/07/0788; GA ČR GAP501/10/1215
Grant - others:University of South Bohemia(CZ) 58/2006/P-BF; University of South Bohemia(CZ) 56/2006/P-BF
Institutional research plan: CEZ:AV0Z50070508; CEZ:AV0Z40550506
Keywords : AKH * lipid * diacylglycerol
Subject RIV: ED - Physiology
Impact factor: 1.989, year: 2010

The diacylglycerols (DG) molecular species and their fatty acid (FA) composition were investigated by electrospray mass spectrometry (ESI-MS) and by gas chromatography with flame ionisation detection (GC-FID) in haemolymph of Locusta migratoria after application of adipokinetic hormones (AKH). The analyses showed (1) a heterogeneous distribution of individual DGs in haemolymph after the AKH application. The results revealed that mobilization of the DGs is specific and that palmitic (C16:0) and oleic acid (C18:1) residues form about 20% of the total mobilized DGs. (2) Analysis of fat body TGs revealed that all Locmi-AKHs mobilize the DGs selectively with the preference of those possessing the C18 and C16 FAs. (3) A distribution of FAs in the DG structures indicated a certain degree of Locmi-AKH selectivity toward the mobilized DGs and hence the FAs. The Locmi-AKH-I significantly prefers mobilization unsaturated, while Locmi-AKH-II and -III mobilization of saturated FAs.
Permanent Link: http://hdl.handle.net/11104/0184376