The effects of light-induced reduction of the photosystem II reaction center

0341236 - ÚVGZ 2010 RIV US eng J - Journal Article
Kutý, Michal … Total 4 authors
The effects of light-induced reduction of the photosystem II reaction center.
Journal of Molecular Modeling. Roč. 8, č. 15 (2009), s. 923-933. ISSN 1610-2940. E-ISSN 0948-5023
R&D Projects: GA MŠMT(CZ) LC06010
Institutional research plan: CEZ:AV0Z60870520
Keywords : Photosystem II * Reaction center * Pheophytin
Subject RIV: CE - Biochemistry

Accumulation of reduced pheophytin a (Pheo-D1) in photosystem II reaction center (PSII RC) under illumination at low redox potential is accompanied by changes in absorbance and circular dichroism spectra. The temperature dependences of these spectral changes have the potential to distinguish between changes caused by the excitonic interaction and temperature-dependent processes. We observed a conformational change in the PSII RC protein part and changes in the spatial positions of the PSII RC pigments of the active D1 branch upon reduction of Pheo-D1 only in the case of high temperature (298 K) dynamics. The resulting absorption difference spectra of PSII RC models equilibrated at temperatures of 77K and 298K were highly consistent with our previous experiments in which light-induced bleaching of the PSII RC absorbance spectrum was observable only at 298K.
Permanent Link: http://hdl.handle.net/11104/0184285