Ubiquity of .i.Polynucleobacter necessarius./i. ssp. .i.asymbioticus./i. in lentic freshwater habitats of a heterogenous 2000 km.sup.2./sup. area

0341201 - BC 2010 RIV GB eng J - Journal Article
Jezberová, Jitka - Jezbera, Jan - Brandt, U. - Lindström, E.S. - Langenheder, S. - Hahn, M.W.
Ubiquity of Polynucleobacter necessarius ssp. asymbioticus in lentic freshwater habitats of a heterogenous 2000 km² area.
Environmental Microbiology. Roč. 12, č. 3 (2010), s. 658-669. ISSN 1462-2912. E-ISSN 1462-2920.
[SAME /11./. Piran, 30.08.09-05.09.09]
R&D Projects: GA ČR(CZ) GA206/08/0015
Institutional research plan: CEZ:AV0Z60170517
Keywords : Polynucleobacter * ubiquity * ecotype
Subject RIV: EE - Microbiology, Virology
Impact factor: 5.537, year: 2010

We sampled stagnant freshwater habitats in a heterogeneous 2000 square kilometre area, together with ecologically different habitats outside this area. In total, 137 lakes, ponds and puddles were investigated, which represent an enormous diversity of habitats differing, e.g. in depth (<10 cm–171 m) and pH (3.9–8.5). PnecC bacteria were detected by cultivation-independent methods in all investigated habitats, and their presence was confirmed by cultivation of strains from selected habitats representing the whole studied ecological range. The determined relative abundance of the subspecies ranged from values close to the detection limit of FISH (0.2%) to 67% (average 14.5%), and the highest observed absolute abundance was 5.3 million cells per ml. Statistical analyses revealed that the abundance of PnecC bacteria was controlled by factors linked to concentrations of humic substances, which support the hypothesis that these bacteria utilize photodegradation products of humic substances.
Permanent Link: http://hdl.handle.net/11104/0184263