Structure of the motor subunit of type I restriction-modification complex EcoR124I

0341137 - ÚVGZ 2010 RIV US eng J - Journal Article
Lapkouski, Mikalai … Total 7 authors
Structure of the motor subunit of type I restriction-modification complex EcoR124I.
Nature Structural & Molecular Biology. Roč. 1, č. 16 (2009), s. 94-95. ISSN 1545-9993. E-ISSN 1545-9985
Institutional research plan: CEZ:AV0Z60870520
Keywords : SWI2/SNF2 ATPASE * DNA * ENDONUCLEASE
Subject RIV: CE - Biochemistry
Impact factor: 12.273, year: 2009

Type I restriction-modification enzymes act as conventional adenine methylases on hemimethylated DNAs, but unmethylated recognition targets induce them to translocate thousands of base pairs before cleaving distant sites nonspecifically. The first crystal structure of a type I motor subunit responsible for translocation and cleavage suggests how the pentameric translocating complex is assembled and provides a structural framework for translocation of duplex DNA by RecA-like ATPase motors.
Permanent Link: http://hdl.handle.net/11104/0184216