Photobioreactor for cultivation and real-time, in-situ measurement of O-2 and CO2 exchange rates, growth dynamics, and of chlorophyll fluorescence emission of photoautotrophic microorganisms

0341130 - ÚVGZ 2010 RIV DE eng J - Journal Article
Červený, Jan - Šetlík, Ivan … Total 4 authors
Photobioreactor for cultivation and real-time, in-situ measurement of O-2 and CO2 exchange rates, growth dynamics, and of chlorophyll fluorescence emission of photoautotrophic microorganisms.
Engineering in Life Sciences. Roč. 3, č. 9 (2009), s. 247-253. ISSN 1618-0240. E-ISSN 1618-2863
R&D Projects: GA ČR(CZ) GA206/05/0894; GA ČR(CZ) GA206/09/1284; GA ČR GA206/08/1683
Institutional research plan: CEZ:AV0Z60870520; CEZ:AV0Z50200510
Keywords : PHOTOSYNTHESIS * Bioreactor * Biotechnology
Subject RIV: CE - Biochemistry
Impact factor: 1.240, year: 2009

A detailed knowledge about the dynamics of phytoplanktonic photosynthesis and respiration is crucial for the determination of primary productivity in oceans as well as for biotechnology. The dynamics are best studied in photobioreactors that are able to simulate natural conditions. We demonstrate performance of a newly designed laboratory photobioreactor that meets these needs, with a study of green alga Scenedesmusquadricauda. The instrument monitored the culture growth by measuring the optical densities at 735 and 680 nm and by measuring several fluorescence parameters. These widely used but rather indirect estimates were confronted with direct measurements of O2 quantum yields. The CO2 fluxes in and out of the culture media as well as the dissolved CO2 in algal suspension were also recorded. The experiments demonstrated potential of the photobioreactor to reveal minute modulations in gas exchange rates as well as to yield data for planning of large scale photobioreactors. A detailed knowledge about the dynamics of phytoplanktonic photosynthesis and respiration is crucial for the determination of primary productivity in oceans as well as for biotechnology. The dynamics are best studied in photobioreactors that are able to simulate natural conditions. We demonstrate performance of a newly designed laboratory photobioreactor that meets these needs, with a study of green alga Scenedesmusquadricauda. The instrument monitored the culture growth by measuring the optical densities at 735 and 680 nm and by measuring several fluorescence parameters. These widely used but rather indirect estimates were confronted with direct measurements of O2 quantum yields. The CO2 fluxes in and out of the culture media as well as the dissolved CO2 in algal suspension were also recorded. The experiments demonstrated potential of the photobioreactor to reveal minute modulations in gas exchange rates as well as to yield data for planning of large scale photobioreactors.
Permanent Link: http://hdl.handle.net/11104/0184209