Rottlerin inhibits ROS formation and prevents NFkappaB activation in MCF-7 and HT-29 cells

0340896 - BFÚ 2010 RIV US eng J - Journal Article
Maioli, E. - Greci, L. - Souček, Karel - Hýžďalová, Martina - Pecorelli, A. - Fortino, V. - Valacchi, G.
Rottlerin inhibits ROS formation and prevents NFkappaB activation in MCF-7 and HT-29 cells.
Journal of Biomedicine and Biotechnology. -, - (2009), s. 1-7. ISSN 1110-7243
R&D Projects: GA ČR(CZ) GA204/07/0834
Grant - others:GA ČR(CZ) GA310/07/0961
Program: GA
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : Rottlerin * NFkappaB * HT-29 cells
Subject RIV: BO - Biophysics
Impact factor: 1.750, year: 2009

Rottlerin, a polyphenol isolated from Mallotus Philippinensis, has been recently used as a selective inhibitor of PKC delta, although it can inhibit many kinases and has several biological effects. The current study confirms the antioxidant property of Rottlerin against the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) in vitro and against oxidative stress induced by H(2)O(2) and by menadione in culture cells. We also demonstrate that Rottlerin prevents TNFalpha-dependent NFkappaB activation in MCF-7 cells and in HT-29 cells transfected with the NFkappaB-driven plasmid pBIIX-LUC, suggesting that Rottlerin can inhibit NFkappaB via several pathways and in several cell types.
Permanent Link: http://hdl.handle.net/11104/0005785