Subunit–subunit interactions are weakened in mutant forms of acetohydroxy acid synthase insensitive to valine inhibition

0340830 - MBÚ 2010 RIV DE eng J - Journal Article
Kyselková, Martina - Janata, Jiří - Ságová-Marečková, M. - Kopecký, J.
Subunit–subunit interactions are weakened in mutant forms of acetohydroxy acid synthase insensitive to valine inhibition.
Archives of Microbiology. Roč. 192, č. 3 (2010), s. 195-200. ISSN 0302-8933. E-ISSN 1432-072X
R&D Projects: GA MŠMT 2B08064
Institutional research plan: CEZ:AV0Z50200510
Keywords : Streptomyces cinnamonensis * Acetohydroxy acid synthase * Subunit-subunit interaction
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.754, year: 2010

In acetohydroxy acid synthase from Streptomyces cinnamonensis mutants aVected in valine regulation, the impact of mutations on interactions between the catalytic and the regulatory subunits was examined using yeast twohybrid system. Mutations in the catalytic and the regulatory subunits were projected into homology models of the respective proteins. Two changes in the catalytic subunit, E139A (domain) and Q217 (domain), both located on the surface of the catalytic subunit dimer, lowered the interaction with the regulatory subunit. Three consecutive changes in the N-terminal part of the regulatory subunit were examined
Permanent Link: http://hdl.handle.net/11104/0183993