Robust Up-Regulation of Nuclear Red Fluorescent-Tagged Fos Marks Neuronal Activation in Green Fluorescent Vasopressin Neurons after Osmotic Stimulation in a Double-Transgenic Rat

0340575 - ÚEM 2010 RIV US eng J - Journal Article
Fujihara, H. - Ueta, Y. - Suzuki, H. - Katoh, A. - Ohbuchi, T. - Otsubo, H. - Dayanithi, Govindan - Murphy, D.
Robust Up-Regulation of Nuclear Red Fluorescent-Tagged Fos Marks Neuronal Activation in Green Fluorescent Vasopressin Neurons after Osmotic Stimulation in a Double-Transgenic Rat.
Endocrinology. Roč. 150, č. 12 (2009), s. 5633-5638. ISSN 0013-7227. E-ISSN 1945-7170
Institutional research plan: CEZ:AV0Z50390512; CEZ:AV0Z50390703
Keywords : transcription * protein fusion gene * hypothalmus
Subject RIV: FH - Neurology
Impact factor: 4.752, year: 2009

The up-regulation in the expression of mRNA or protein encoded by the c-fos gene is widely used as a marker of neuronal activation elicited by various stimuli. To facilitate the detection of activated neurons, we generated transgenic rats expressing a fusion gene consisting of c-fos coding sequences in frame with monomeric red fluorescent protein 1 (mRFP1) under the control of c-fos gene regulatory sequences (c-fos-mRFP1 rats). In these double-transgenic rats, almost all eGFP neurons in the supraoptic nucleus and PVN expressed nuclear mRFP1 fluorescence 90 min after hypertonic saline administration. Furthermore, when combined with transgenes expressing another fluorophore under the control of cell-specific regulatory sequences, activation of specific neuronal cell types in response to physiological cues can be readily detected.
Permanent Link: http://hdl.handle.net/11104/0183787