The effect of laparotomy on hydroxyl radicals, singlet oxygen and antioxidants measured by EPR method in the tails of rats

0340387 - ÚACH 2010 RIV SE eng J - Journal Article
Fricová, J. - Stopka, Pavel - Křížová, Jana - Yamamotová, A. - Rokyta, R.
The effect of laparotomy on hydroxyl radicals, singlet oxygen and antioxidants measured by EPR method in the tails of rats.
Neuroendocrinology Letters. Roč. 30, č. 3 (2009), s. 373-376. ISSN 0172-780X. E-ISSN 2354-4716
Institutional research plan: CEZ:AV0Z40320502
Keywords : EPR-direct measurement in anesthetized rats * laparotomy * visceral pain
Subject RIV: ED - Physiology
Impact factor: 1.047, year: 2009

The aim of the study was to demonstrate that direct measurement of hydroxyl radicals and singlet oxygen in the tail of living rats is possible. The basic level of hydroxyl radicals and singlet oxygen were measured and the effects of antioxidants on their levels were studied in the tail of living anaesthetized rats after acute postoperative pain. Laparotomy was performed as the source of acute abdominal pain. After closure of the abdominal cavity, the animals began to awaken within 30-60 minutes. They were left to recover for 2-3 hours; then they were reanesthetized and the effect of antioxidants was measured on the numbers of hydroxyl radicals and singlet oxygen via blood in the tail.
Permanent Link: http://hdl.handle.net/11104/0183639