Reactive Landing of Gas-Phase Ions as a Tool for the Fabrication of Metal Oxide Surfaces for In Situ Phosphopeptide Enrichment

0340264 - MBÚ 2010 RIV US eng J - Journal Article
Blacken, G. R. - Volný, Michael - Diener, M. - Jackson, K. E. - Ranjitkar, P. - Maly, D. J. - Tureček, F.
Reactive Landing of Gas-Phase Ions as a Tool for the Fabrication of Metal Oxide Surfaces for In Situ Phosphopeptide Enrichment.
Journal of the American Society for Mass Spectrometry. Roč. 20, č. 6 (2009), s. 915-926. ISSN 1044-0305. E-ISSN 1879-1123
Institutional research plan: CEZ:AV0Z50200510
Keywords : TANDEM MASS-SPECTROMETRY * SELECTIVE DETECTION * PHOSPHOPROTEOME ANALYSIS
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.391, year: 2009

Zirconium, titanium, and hafnium oxide-coated stainless steel surfaces are fabricated by reactive landing of gas-phase ions produced by electrospray ionization of group IVB metal alkoxides. The Surfaces are used for in situ enrichment of phosphopeptides before analysis by matrix-assisted laser desorption ionization (MALDI) mass spectrometry. To evaluate this method we characterized ZrO2, (zirconia) Surfaces by (1) comparison with the other group IVB metal oxides of TiO2, (titania) and HfO2, (hafnia), (2) morphological characterization by SEM image analysis, and (3) dependence of phosphopeptide enrichment on the metal oxide layer thickness
Permanent Link: http://hdl.handle.net/11104/0183544