Automated Ambient Desorption-Ionization Platform for Surface Imaging Integrated with a Commercial Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

0340257 - MBÚ 2010 RIV US eng J - Journal Article
Pól, Jaroslav - Vidová, Veronika - Kruppa, G. - Kobliha, Václav - Novák, Petr - Lemr, Karel - Kotiaho, T. - Kostiainen, R. - Havlíček, Vladimír - Volný, Michael
Automated Ambient Desorption-Ionization Platform for Surface Imaging Integrated with a Commercial Fourier Transform Ion Cyclotron Resonance Mass Spectrometer.
Analytical Chemistry. Roč. 81, č. 20 (2009), s. 8479-8487. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA MŠMT LC07017
Institutional research plan: CEZ:AV0Z50200510
Keywords : ATMOSPHERIC-PRESSURE PHOTOIONIZATION * COMPREHENSIVE CLASSIFICATION-SYSTEM * mass spectrometry
Subject RIV: EE - Microbiology, Virology
Impact factor: 5.214, year: 2009

A fully automated atmospheric pressure ionization platform has been built and coupled with a commercial high-resolution Fourier transform ion cyclotron resonance mass spectrometer (FTICR-MS) instrument. The outstanding performance of this instrument allowed screening on the basis of exact masses in imaging mode. The main novel aspect was in the integration of the atmospheric pressure ionization imaging into the current software for matrix-assisted laser desorption ionization (MALDI) imaging, which allows the user of this commercial dual-source mass spectrometer to perform MALDI-MS and different ambient MS imaging from the same user interface and to utilize the same software tools. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) were chosen to test the ambient surface imaging capabilities of this new ionization platform
Permanent Link: http://hdl.handle.net/11104/0183538